Use of Aperio Whole Slide Digital Imaging and Image Analysis for Pre-Clinical Trial Studies

Victoria Sefcsik Indiana University-Purdue University Indianapolis
Faculty Sponsor(s): George Sandusky Indiana University-Purdue University Indianapolis
Aperio Imaging provides customizable imaging techniques that are becoming an industry standard today. Image analysis with Aperio has the ability to quantify area and cells by identifying colors according to stain and intensity in order to quantify. Aperio can perform whole slide digital scanning that takes approximately 2.5 minutes per slide and can capture data for imaging in the 2-40x range. Aperio Imaging can be used with H&E, Trichrome, and PAS stains. Data is generated using the Aperio positive pixel software using algorithms to assign percentages to levels of positive staining in comparison to the entire area. Analysis can be completed using the entire image or by choosing hotspot areas (typically three to five) that accurately represent the tissue as a whole. In this study, aging mouse tissue samples were evaluated using Aperio positive pixel software to analyze percent tumor in kidney and lung H&E slides. In conclusion, the positive pixel algorithm was successful in identifying tumor cells using H&E slides.
Biochemistry & Molecular Biology
Poster Presentation

When & Where

Irwin Library 3rd Floor