Peroxidase Activity and Signaling Molecules in Moss Defense Pathways

Tyler Schenck Butler University
Faculty Sponsor(s): Phil Villani Butler University
While pathogens have evolved complex pathways for the infection of plant material, plants have also needed to develop pathways to respond and defend to these threats. A part of this defense response is known as an oxidative burst in which the plant release oxidative radicals and hydrogen peroxide. Hydrogen peroxide is involved in multiple mechanisms for plant defense including chemical changes in the cell wall and signaling for selective necrosis to stymie fungal growth. For this project, the goal was to evaluate the intensity and timing of chemical and enzymatic responses by using colorimetric assays to quantify both hydrogen peroxide and a class of plant enzymes that is known to release hydrogen peroxide and break it down in different environmental conditions. These responses within the cell and secretions evaluated over time. A secondary goal was to observe this pathway in model organisms for both acrocarp and pleurocarp growth forms of mosses. This would provide some information on the necessity of ground cover plants to have a different response than other plants with apical growth forms.
Oral Presentation

When & Where

10:30 AM
Gallahue Hall 102