NASH and Aperio Positive Pixel Quantification for Type II Diabetes on FATZO Mouse Model

Courtney Finnearty Indiana University-Purdue University Indianapolis
Faculty Sponsor(s): George Sandusky Indiana University-Purdue University Indianapolis
More than 86 million adults in the United States have blood sugar levels higher than normal but not high enough to diagnose Type II diabetes. Without weight loss and physical activity, 15% to 30% of these people will develop Type II diabetes within five years. Early diagnosis of pre-stage diabetes may reduce development of Type II diabetes. This study analyzed two quantification methods, NASH score and Aperio Positive Pixel Quantification, throughout the development of Type II Diabetes in the FATZO mouse model. Two groups of mice which differentiated by Western diet and control diet were analyzed throughout the course of 4 months. With the Western diet, the NASH score revealed a positive correlation with the severity of steatosis, lobular inflammation, and fibrosis. Ballooning degeneration showed a negative correlation. With the control diet, the NASH score showed a negative correlation with steatosis, lobular inflammation, ballooning degeneration, and fibrosis. The Aperio Positive Pixel Quantification was performed for fibrosis development with Sirius Red and Trichrome immunostains in the FATZO mouse livers. Trichrome showed a consistent fibrosis positivity score in the Western diet and showed an inconsistent fibrotic positivity score for control diet. Sirius Red showed a slight positive correlation of fibrosis with Western diet and a slight negative correlation of fibrosis for the control diet. The Aperio Positive Pixel Quantification was limited to fibrotic tissue due to the immunostain chosen. NASH score is the most comprehensive method of analysis for type II diabetes development in liver specimens.
Biochemistry & Molecular Biology
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Irwin Library 3rd Floor