The Effects of Salicylic Acid on the Hypersensitive Response of Physcomitrella patens

Matthew Hathaway Butler University
Faculty Sponsor(s): Philip Villani Butler University
Reactive Oxygen Species (ROS) were originally believed to be toxic to plants and induce apoptosis in cells. New findings have led to the suggestion that ROS act as a secondary signaling pathway for cells and play an important role in generating a hypersensitive response (HR), which is crucial for dealing with cellular infections. The hypersensitive response will lead to a plantwide immune response known as an systemic acquired response (SAR). This discovery warranted further study by observing how plants, specifically mosses, generate ROS in the presence of hormones like salicylic acid (SA). Elevated levels of hydrogen peroxide indicated that a hypersensitive response was occurring when P. patens was placed in SA solution. Using a colorimetric assay, we were able to quantify hydrogen peroxide levels and establish an elevated response curve. This curve showed that there were statistically significant levels of hydrogen peroxide present at several instances during treatment. Utilizing PCR, we were able to see if there was any variance in the gene expression of P. patens species exposed to SA solution.
Oral Presentation

When & Where

10:30 AM
Gallahue Hall 106