Kat Morris Saint Mary's College
Faculty Sponsor(s): Don Paetkau Saint Mary's CollegePaper Analytical Devices (PADs) are an inexpensive and efficient method for the detection of Substandard, Spurious/Falsely-labeled/Falsified/Counterfeit (SSFFC) drugs. PADs are particularly useful in low resource areas where pharmaceutical regulation of SSFFC drugs may be a challenge. The purpose of this project was to develop a PAD using yeast as a biosensor to detect for the presence of prednisone. The glucocorticoid receptor expressing pRR-GR-5Z plasmid was obtained from Addgene. The Lac-Z reporter gene was used to detect prednisone with a visual color change. A 96 well assay was performed with ONPG and beta X-gal buffer to determine if the presence of prednisone would induce a color change. Dilutions were performed to determine the best concentration of yeast to cause a uniform color change. After a range of dilutions were performed, the 96 well assays containing the yeast with the GR plasmid were induced with prednisone. The ONPG buffer had more consistent results when compared to the beta X-gal buffer, but it was determined that the yellow color was not suitable for a paper device. The beta X-gal buffer produced blue color, but it requires cell lysis using liquid nitrogen, which is not suitable for field testing. The secreted alpha X-gal reporter may be a viable alternative to eliminate the requirement for liquid nitrogen.
Biochemistry & Molecular Biology
When & Where
Irwin Library 3rd Floor