32. Screening Drug Library in Combination with Albendazole in C. elegans

Ashley Grabowski Bethel College
Faculty Sponsor(s): Brian Ellis Bethel College
Over 1.5 billion people are infected with soil-transmitted helminths (STHs), which are intestinal parasitic nematodes. The majority of those affected by these nematodes are the impoverished, especially the women and children. These parasitic nematodes not only affect a substantial number of people, but they also have an extensive financial toll on livestock. Transmitted through contaminated soil, these nematodes include the large round-worm (Ascaris lumbricoides), whipworm (Trichuris trichiura), and hookworm (Ancylostoma ceylancium, Ancylostoma duodenale, and the Necator americanus). Currently four anthelminitic drugs are recognized by the WHO for mass drug administration (MDA). Albendazole is the preferred drug out of the four approved, but a resistance to it has developed. Because of the severity of this problem, MDA programs have been trying to control or eradicate human parasitic nematodes. Due to the development and distribution of resistance in parasitic nematodes, a new therapy for intestinal parasitic nematodes is in critical need. In response to this need, new drugs are being discovered. Partridge et al. screened the Pathogen Box library obtained from the Medicines for Malaria Venture and found the top 20 hit compounds out of the 400 drugs. Those 20 drugs were used in a secondary screen where 18 of them were found to be active. In this study, the drugs from the Pathogen Box were combined with albendazole to observe their effect on Caenorhabditis elegans (C. elegans), a model organism for anthelmintic testing. The drugs were introduced to the C. elegans during the L4 stage and were screened daily for significant responses. This includes effect on growth, motility, phenotypes, or any observable trend. Throughout this study, we predict that when combined with albendazole, which has a known effect, diverse classes of molecules had a range of increased effects on C. elegans. Using the data collected, it is projected that one of these classes of molecules could produce an effective treatment for intestinal parasitic nematodes.
Poster Presentation

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Irwin Library 3rd Floor