20. Trace Metal Analysis of Male and Female Periodical Cicadas using Microwave Digestion and Inductively Coupled Plasma-Atomic Emission Spectrometry

Andrew Seiler Thomas More College
Faculty Sponsor(s): William Wetzel Thomas More College
Periodical cicadas are several species of cicadas that experience 13- or 17-year life cycles. The greatest portion of these cycles are spent as nymphs underground consuming the roots of plants. Near the end of their lifetime, cicadas emerge in large masses in localized geographical regions. Because they both prey on plant species while they are under ground and are prey to birds, reptiles, insects, and other predators after emergence, periodical cicadas are situated among the middle of the food chain. As such, cicadas are potentially an interesting bioindicator species. In this study, periodical cicadas were collected from Southwestern Ohio and Northern Kentucky in 2008, 2014, and 2017. The insects were dried to a constant mass, digested using microwave digestion, and metal levels were determined using inductively coupled plasma-atomic emission spectroscopy (ICP-AES). Trace-metals present in males and females of the species Magicicada cassini at a specific location and at multiple collection sites were compared. Results of this study suggest there may be subtle differences in trace-metal composition between males and females at a specific location; however, there does not appear to be patterned trace-metal differences across different broods or geographic locations.
Poster Presentation

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Irwin Library 1st Floor