2. Detecting and Differentiating Pseudomonas species Within Soil, Water, and Plant Matter Following a Multiplex PCR Protocol

Courtney George Midway University
Faculty Sponsor(s): Cynthia Ryder Midway University
Pseudomonas is a genus of gram negative bacteria that are often found within soil, plant matter, and water. There are a large number of sub-species of Pseudomonas, but the current study focuses on Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, and Pseudomonas syringae. The purpose of the current study is to identify the species of a collection of environmental isolates previously determined to be in genus Pseudomonas. The samples were taken from soil, plant matter, and water. Bacterial cultures, DNA extraction, multiplex PCR, and DNA visualization were used to identify each sample. The multiplex PCR utilized can detect Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, and Pseudomonas syringae. From the results of this multiplex PCR, the current study concludes that samples from all four species of Pseudomonas were able to be detected, but some samples did not test into any of the species that were included in the assay.
Biochemistry & Molecular Biology
Poster Presentation

When & Where

Irwin Library Lower Level